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Registro Completo |
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
14/12/2021 |
Data da última atualização: |
14/12/2021 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
KOMMERS, D. R.; BIANCHI, C. A. M.; AVILA, C. A. de J.; HAMPEL, B. J.; SCHIAVO, J.; DALMAGO, G. A. |
Afiliação: |
DANIELA REGINA KOMMERS, Aluna do Curso de Agronomia do Departamento de Estudos Agrários da UNIJUÍ, Bolsista PIBIC/UNIJUÍ, Ijuí-RS, danielakommers@gmail.com; CLEUSA ADRIANE MENEGASSI BIANCHI, Professora orientadora doutora, do Departamento de Estudos Agrários (DEAg)/ UNIJUÍ, Ijuí-RS, cleusa.bianchi@unijui.edu.br; CILENE FÁTIMA DE JESUS AVILA, Aluna do Curso de Agronomia do Departamento de Estudos Agrários da UNIJUÍ, Ijuí-RS, cilene.avila1@gmail.com; BRENDA JACOBOSKI HAMPEL, Aluna do Curso de Agronomia do Departamento de Estudos Agrários da UNIJUÍ, Bolsista PROBIC/FAPERGS, Ijuí-RS, brenda.hampel@hotmail.com; JORDANA SCHIAVO, Engenheira Agrônoma do Instituto Regional de Desenvolvimento Rural (IRDeR) Augusto Pestana - RS, jordana.schiavo@unijui.edu.br; GENEI ANTONIO DALMAGO, CNPT. |
Título: |
Efeito dos arranjos de plantas de canola sobre a produtividade de grãos, componentes de rendimento e teor de óleo. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 28.; SALÃO DO CONHECIMENTO, 2020, Ijuí. Anais... Ijuí: UNIJUI, 20 a 23 out. 2020. |
Idioma: |
Português |
Conteúdo: |
A canola (Brassica napus) teve seu desenvolvimento através do melhoramento genético da colza, uma espécie oleaginosa, pertencente à família Brassicae (crucíferas) a partir da qual foram selecionados cultivares com teores de glucosinolatos e ácido erúcico reduzidos, pois os mesmos são nocivos ao organismo animal (ESTEVEZ et al., 2014). Os genótipos de canola disponíveis para o cultivo apresentam grande variabilidade de produção entre anos, além disso, a canola é altamente plástica (TOMM et al., 2007), sendo necessário estudos para identificar as melhores condições de manejo, visando atingir a produtividade potencial da espécie e adequar os arranjos de semeadura para cada genótipo. |
Palavras-Chave: |
Brassica napus L; Oleaginosas; Var oleífera. |
Thesagro: |
População. |
Thesaurus Nal: |
Oilseeds; Population. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229093/1/2020-PA-VI-IJUI-Arranjo-de-Plantas-Efeito-do-Arranjo-Kommers-Resumo.pdf
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Marc: |
LEADER 01514nam a2200241 a 4500 001 2137747 005 2021-12-14 008 2020 bl uuuu u00u1 u #d 100 1 $aKOMMERS, D. R. 245 $aEfeito dos arranjos de plantas de canola sobre a produtividade de grãos, componentes de rendimento e teor de óleo.$h[electronic resource] 260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 28.; SALÃO DO CONHECIMENTO, 2020, Ijuí. Anais... Ijuí: UNIJUI, 20 a 23 out. 2020.$c2020 520 $aA canola (Brassica napus) teve seu desenvolvimento através do melhoramento genético da colza, uma espécie oleaginosa, pertencente à família Brassicae (crucíferas) a partir da qual foram selecionados cultivares com teores de glucosinolatos e ácido erúcico reduzidos, pois os mesmos são nocivos ao organismo animal (ESTEVEZ et al., 2014). Os genótipos de canola disponíveis para o cultivo apresentam grande variabilidade de produção entre anos, além disso, a canola é altamente plástica (TOMM et al., 2007), sendo necessário estudos para identificar as melhores condições de manejo, visando atingir a produtividade potencial da espécie e adequar os arranjos de semeadura para cada genótipo. 650 $aOilseeds 650 $aPopulation 650 $aPopulação 653 $aBrassica napus L 653 $aOleaginosas 653 $aVar oleífera 700 1 $aBIANCHI, C. A. M. 700 1 $aAVILA, C. A. de J. 700 1 $aHAMPEL, B. J. 700 1 $aSCHIAVO, J. 700 1 $aDALMAGO, G. A.
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Embrapa Trigo (CNPT) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
27/10/2015 |
Data da última atualização: |
30/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, CPATU; CLARA SLADE OLIVEIRA, CNPGL; Cláudia Lima Verde Leal, USP; Marina Ragagnin de Lima, UNESP; Maite Del Collado, UNESP / USP; Roberta Vantini, UNESP; Fabio Morato Monteiro, Centro APTA Bovinos de Corte, Instituto de Zootecnia; SIMONE CRISTINA MEO NICIURA, CPPSE; Joaquim Mansano Garcia, UNESP. |
Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, Dec. 2015. |
DOI: |
http://dx.doi.org/10.1017/S0967199414000537 |
Idioma: |
Inglês |
Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
Thesaurus NAL: |
chromatin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02641naa a2200289 a 4500 001 2027313 005 2022-05-30 008 2015 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1017/S0967199414000537$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource] 260 $c2015 520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. 650 $achromatin 653 $aBovine 653 $aChemically induced enucleation 653 $aMicrotubule 653 $aNuclear transfer 700 1 $aOLIVEIRA, C. S. 700 1 $aLEAL, C. L. V. 700 1 $aLIMA, M. R. de 700 1 $aCALLADO, M. del 700 1 $aVANTINI, R. 700 1 $aMONTEIRO, F. M. 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tZygote$gv. 23, n. 6, p. 852-862, Dec. 2015.
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